Our peptide synthesizer is a laboratory instrument used for the synthesis of peptide.
Volume: 100ml, 200ml, 500ml, 1L, 2L, 3L, 5L, 10L, 20L, 30L, 50L, 100L, form laboratory scale to pilot plant and industrial scale.
Vessel: Single wall, double wall or triple wall available
Working pressure: Full vacuum to 0.05/0.1Mpa
Working temperature: -80°C ~ +220°C
Filter device: PTFE, glass or stainless-steel, pore size 0.1um to 200um
Peptides are short chains of amino acids linked by peptide bonds, and they play crucial roles in biological processes, drug development and research.
Peptide synthesis equipment simplifies and automates the complex process of peptide synthesis, making it faster, more efficient.
The most common method employed by peptide synthesizer is solid-phase peptide synthesis(SPPS).
What is Solid-Phase Peptide Synthesis(SPPS) ?
Solid-phase synthesis is a common technique for peptide synthesis. Usually, peptides are synthesised from the carbonyl group side (C-terminus) to amino group side (N-terminus) of the amino acid chain in the SPPS method, although peptides are biologically synthesised in the opposite direction in cells. In peptide synthesis, an amino-protected amino acid is bound to a solid phase material or resin (most commonly, low cross-linked polystyrene beads), forming a covalent bond between the carbonyl group and the resin, most often an amido or an ester bond. Then the amino group is deprotected and reacted with the carbonyl group of the next N-protected amino acid. The solid phase now bears a dipeptide. This cycle is repeated to form the desired peptide chain. After all reactions are complete, the synthesised peptide is cleaved from the bead.
The protecting groups for the amino groups mostly used in the peptide synthesis are 9-fluorenylmethyloxycarbonyl group (Fmoc) and t-butyloxycarbonyl (Boc). A number of amino acids bear functional groups in the side chain which must be protected specifically from reacting with the incoming N-protected amino acids. In contrast to Boc and Fmoc groups, these have to be stable over the course of peptide synthesis although they are also removed during the final deprotection of peptides.
Information from WikipediA.
Key Components of a Peptide Synthesizer
1. Control System
- It automates the peptide synthesis, ensuring precise timing and sequencing of each step.
2. Reagent Delivery System
- This systems include pumps, valves and tubing to deliver amino acids, coupling agents, deprotecting agents and solvents to the reaction vessel. It can ensure accurate delivery of reagents at each step.
3. Reaction Vessel
- Our reaction vessel is jacketed design, it holds the solid-phase resin and reaction mixture, designed to faciliate efficient mixing and optimal contact between reagents and the solid support. It also equip with mechanism stirrer to ensure efficient mixing.
4. Temperature Control
- The jacket vessel design and an external temperature control unit to maintain the appropriate temperature for many peptide synthesis reactions.
5. Drainage Channels
- Our drainage channels work efficiently to remove spent reagents and by-products from the reaction vessel to waste containers to collect and dispose of liquid waste.
6. Monitoring and Sensors
- Our monitoring and sensors include temperature sensors, pressure sensors and sometimes pH sensors. They continuously monitor reaction conditions to ensure optimal synthesis parameters.
Peptide Synthesizer Structure
Control system of peptide synthesizer, control mixing speed, sensors and reagent delivery system.
Reagent Delivery System include pumps and valves, ensure accurate delivery of reagents at each step.
Reaction Vessel holds the solid-phase resin and reaction mixture.
Temperature Control Unit provide circulating heating or cooling medium for reaction need
Draiange chnnels for removing spent reagents and by-products
Customized function ports such as liquid feeding, solid feeding and sensors port
Lifting structure allow filter device maintance and cleaning, easy unloading the solid resin
Waste container to collect and dispose of liquid waste under vacuun condition