A Solid Phase Peptide Synthesis (SPPS) Vessel is a specialized reaction container designed to perform Solid Phase Peptide Synthesis – the standard method for chemically creating peptides (short chains of amino acids) step-by-step on an insoluble solid support.
Volume: 100ml,200ml, 500ml, 1L, 2L, 3L, 5L, 10L, 20L, 30L, 50L, 100L, for the laboratory scale, pilot plant and big industrial production scale. please refer our laboratory nutsche filter and industrial nutsche filter.
Vessel: Single wall, double wall or triple wall available
Working pressure: Full vacuum to 0.05Mpa
Working temperature: -80°C ~ +220°C
Filter device: PTFE, glass or stainless-steel sand core plate with pore size 0.1um to 200um and PTFE filter plate with filter cloth structure.
In essence, an SPPS Vessel is the reactor, mixer, and filter combined into one purpose-built container, enabling the repetitive chemical steps of peptide synthesis while securely holding the solid support throughout the entire process.
Its efficient filtration and inert environment are critical for successful synthesis.
Working Principle
1.Resin Loading
- The synthesis begins with loading the first amino acid onto the resin support. The amino acid is typically protected to prevent unwanted reactions.
2. Deprotection
- The protective group on the amino acid is removed to expose its reactive amine group. This step prepares the amino acid for coupling with the next amino acid.
3. Amino Acid Coupling
- The next amino acid, also protected, is activated using a coupling agent and then added to the reaction vessel. The activated amino acid reacts with the exposed amine group on the resin-bound amino acid, forming a peptide bond.
4. Repetition
- The cycle of deprotection and coupling is repeated for each subsequent amino acid in the desired peptide sequence. The machine precisely controls each step to ensure high yield and purity.
5. Cleavage and Purification
- Once the entire peptide sequence is synthesized, the peptide is cleaved from the resin support and deprotected. The crude peptide is then purified, typically using high-performance liquid chromatography(HPLC) or other purification methods.
Structure of Solid Phase Peptide Synthesis Vessel
Our full Jacket vessel allow temperature control of small volume of reaction material
Customized inlet ports on glass overhead to meet specific process requirement
3-way discharge valve allow bubbling and nitrogen purge
Liftable trolley support unload solid resins